甘草酸二铵联用红花注射液对大鼠溃疡性结肠炎炎症的影响
【出 处】:
【作 者】:
【摘 要】AIM: To evaluate the therapeutic effects of diam- monium glycyrrhizinate (DG) combined with safflower solution (SS) on experimental colitis and on the expression of interleukin-8 (IL-8) in rats. METHODS: Forty Sprague-Dawley rats were randomized into a normal control group, a treatment group (DG combined with SS), a DG group and a TNBS control group, with 10 rats in each group. Colitis was induced by rectal administration of TNBS in rats in the treatment group, DG group and TNBS control group. Rats in the treatment group were treated with DG (40 mg/kg) and SS (400 mg/kg) every day, and the DG group was treated with DG (40 mg/kg) every day. Disease activity index (DAI) and his- tological damage score were calculated, and the expression of IL-8 in colon tissues was detected by immunohistochemistry. RESULTS: In the normal control group, treat- ment group, DG group and TNBS control group, DAIs were 1.4 ± 0.3, 2.4 ± 0.2, 3.8 ± 0.4 and 7.6 ± 0.9, respectively; histological damage scores were 1.5 ± 0.4, 2.4± 0.4, 3.7 ± 0.6 and 6.3 ± 1.1, re- spectively; and the positive percentages of IL-8 were 15.7% ± 3.7%, 20.8%± 4.7%, 34.7% ± 4.9% and 56.4%± 6.9%, respectively. Compared with the TNBS control group, DAI and histological injury scores were significantly improved in the treatment group and DG group, and the expres- sion of IL-8 was decreased significantly in the treatment group (P 〈 0.01 or 〈 0.05). Compared with the DG group, DAI and histological dam- age scores were significantly improved and the expression of IL-8 was decreased significantly in the treatment group (P 〈 0.01 or 〈 0.05). CONCLUSION: DG combined with SS exerts a better effect than DG alone in the treatment of experimental ulcerative colitis in rats, and the underlying mechanism is possibly associated with affecting the expression of IL-8.
相关热词搜索: 甘草酸二铵 红花注射液 溃疡性结肠炎炎症 治疗
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