内外源性GAS对二甲基肼诱导的大鼠大肠癌变中EGF和PGE_2表达的影响
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【摘 要】目的:探讨内外源性胃泌素(gastrin,GAS)对二基肼诱导大鼠大肠癌变中表皮生长因子(epidermal growth factor,EGF)、前列腺素E2(prostaglandin E2,PGE2)表达的影响以及GAS、环氧酶(cyclooxyge-nase,COX)-2、EGF、PGE2、表皮生长因子受体(epidermal growth factor receptor,EGFR)在大肠癌中的作用.方法:140只大鼠随机分为7组:(1)二甲基肼(1,2-dimethyl hydrazine,DMH)+GAS;(2)DMH+奥美拉唑肠溶胶囊(omeprazole entericcapsules,PPI);(3)DMH;(4)DMH+GAS+丙谷胺(proglumide,PGL);(5)DMH+PPI+PGL;(6)DMH+PGL;(7)对照组.放射免疫法检测血清和大肠组织匀浆上清液的GAS、EGF和PGE2浓度,免疫组织化学法检测大肠组织中的COX-2和EGFR的表达,做图象光密度分析.结果:(1)第18周时各组血清和组织中GAS、EGF、PGE2浓度比较:GAS:DMH+GAS(15.59pg/mL±2.90pg/mL,0.38pg/mL±0.11pg/mL)组与DMH+GAS+PGL(15.31pg/mL±5.66pg/mL,0.35pg/mL±0.10pg/mL)组均高于对照组(8.64pg/mL±2.36pg/mL,0.16pg/mL±0.03pg/mL)(P〈0.05),DMH+PPI(20.50pg/mL±3.71pg/mL,0.45pg/mL±0.13pg/mL)组与DMH+PPI+PGL(19.90pg/mL±5.10pg/mL,0.37pg/mL±0.11pg/mL)组均高于DMH(13.12pg/mL±3.47pg/mL,0.19pg/mL±0.04pg/mL)组、DMH+PGL(11.45pg/mL±5.13pg/mL,0.20pg/mL±0.05pg/mL)组及对照组.EGF:DMH+GAS(4.26ng/mL±0.92ng/mL,0.011ng/mL±0.005ng/mL)组与DMH+GAS+PGL(4.29ng/mL±0.50ng/mL,0.009n g/m L±0.005n g/m L)组均高于对照组(2.91ng/mL±0.54ng/mL,0.002ng/mL±0.0007ng/mL)(P〈0.05),DMH+PPI(5.20ng/mL±1.03ng/mL,0.015ng/mL±0.007ng/mL)组与DMH+PPI+PGL(5.13ng/mL±0.50ng/m L,0.011n g/m L±0.007n g/m L)组均高于DMH(3.76ng/mL±1.47ng/mL,0.004ng/mL±0.002ng/mL)组、DMH+PGL(3.59ng/mL±1.12ng/mL,0.002ng/mL±0.0018ng/mL)组及对照组.PGE2:DMH+GAS(76.03pg/mL±60.75pg/mL,2.74pg/mL±0.76pg/mL)及DMH+PPI(70.29pg/mL±66.58pg/mL,2.42pg/mL±0.89pg/mL)组高于其他组,但差异无�